Anti-OTUD6B / OTU domain-containing deubiquitinase 6B

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. OTUD6B antibody detects OTU domain-containing deubiquitinase 6B, a cysteine protease encoded by the OTUD6B gene located on chromosome 8q21.3. OTUD6B belongs to the ovarian tumor (OTU) domain-containing deubiquitinase family, which removes ubiquitin moieties from target proteins to regulate their stability, localization, and signaling activity. OTUD6B plays an important role in cellular protein homeostasis, DNA damage repair, and immune signaling by fine-tuning ubiquitin-mediated pathways. It is expressed in most tissues, with highest levels in brain, liver, and testis, reflecting its broad regulatory functions.Structurally, OTUD6B contains an N-terminal OTU catalytic domain characterized by a conserved cysteine, histidine, and aspartate catalytic triad responsible for cleaving ubiquitin from substrates. It also includes regions for substrate recognition and potential regulatory phosphorylation sites. OTUD6B belongs to the OTU-type deubiquitinase family, which exhibits linkage-specific cleavage of polyubiquitin chains. Co-localization studies show OTUD6B primarily in the cytoplasm but also associated with the nucleus under stress conditions, suggesting roles in both cytosolic and nuclear signaling pathways.Functionally, OTUD6B regulates protein degradation and signaling by removing ubiquitin from specific substrates targeted for proteasomal or lysosomal turnover. It modulates the stability of signaling mediators such as AKT, p53, and components of the mTOR pathway. OTUD6B also contributes to endoplasmic reticulum stress responses and DNA damage repair by reversing ubiquitination on repair factors. In immune cells, OTUD6B helps maintain homeostasis by controlling NF-?B activation and cytokine signaling. Known biochemical activities include cleavage of Lys48- and Lys63-linked ubiquitin chains, thereby influencing protein degradation and signaling transduction.Deficiency or mutation of OTUD6B has been associated with intellectual developmental disorders, craniofacial abnormalities, and growth retardation, collectively known as OTUD6B-related neurodevelopmental syndrome. Dysregulation of OTUD6B expression has also been linked to tumor progression through effects on apoptosis and metabolic control. Pathway associations include ubiquitin-dependent protein catabolism, mTOR signaling, and cellular stress response. During development, OTUD6B supports cell growth and neuronal maturation by regulating key ubiquitinated targets involved in metabolism and transcription.The OTUD6B antibody from NSJ Bioreagents is an excellent reagent for research on deubiquitination mechanisms, proteostasis, and signal transduction regulation.