Anti-Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like)

Periostin, a disulfide-linked 90kD, 811aa protein was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1 (TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml Detects ~1ng/well of rhOSF-2. Cross-reactivity with rmOSF-2 ~45% . , Western Blot: 0.1-0.2ug/ml Detects ~25ng/lane of rhOSF-2 under non-reducing and reducing conditions. , Optimal dilutions to be determined by the researcher. Endotoxin: < 0.1EU/ug (LAL) , Storage and Stability:, Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.