Anti-Opticin (Oculoglycan)

Opticin (oculoglycan) is a member of the small leucine-rich proteoglycan (SLRP) family with homology to class III extracellular matrix family members epiphycan and mimecan. It forms a 90kD non-covalent dimer that is a component of vitreous collagen fibrils in the eye and is also expressed in the retina, iris, ligaments and skin. Mature human opticin shares 73% and 65% aa identity with mouse and rat, respectively. Mutations of opticin have been associated with age-related macular degeneration. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold., ELISA (Direct): 0.5-1ug/ml The detection limit is ~2ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.