Anti-OMA1 / Overlapping with the m-AAA protease 1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. OMA1 antibody recognizes Metallopeptidase OMA1, a zinc-dependent mitochondrial protease that plays a critical role in regulating mitochondrial dynamics and quality control. OMA1 cleaves OPA1, an inner mitochondrial membrane GTPase, under stress conditions such as loss of membrane potential or proteotoxic stress. This cleavage promotes mitochondrial fission, facilitating the removal of damaged mitochondria through mitophagy. The OMA1 antibody is widely used in research on mitochondrial homeostasis, apoptosis, and neurodegenerative disease mechanisms that involve disrupted mitochondrial morphology.OMA1 is encoded by the OMA1 gene located on human chromosome 1p32.2. It encodes an integral membrane protein of the inner mitochondrial membrane, with its catalytic domain facing the intermembrane space. The protein contains a conserved HEXXH metalloprotease motif required for zinc ion coordination and proteolytic activity. OMA1 exists in inactive and active forms that are regulated by mitochondrial potential and proteolytic turnover. Activation leads to processing of long isoforms of OPA1 into short forms, promoting mitochondrial fragmentation during stress.The OMA1 antibody is valuable for investigating mitochondrial stress pathways and proteolytic processing events. Western blot analysis typically detects bands near 60-65 kDa, corresponding to the precursor and active forms of OMA1. In immunocytochemistry, the antibody reveals punctate mitochondrial staining, confirming localization within the inner membrane network. Functional studies using OMA1-deficient cells demonstrate its essential role in maintaining mitochondrial morphology and preventing accumulation of dysfunctional organelles. Loss of OMA1 activity has been associated with neurodegeneration, cardiomyopathy, and metabolic syndromes due to impaired mitochondrial adaptation.OMA1 acts in concert with other mitochondrial proteases, including YME1L1 and PARL, to fine-tune the balance between fusion and fission events. It also participates in the mitochondrial unfolded protein response and contributes to stress-induced apoptosis. NSJ Bioreagents offers a validated OMA1 antibody optimized for western blot, immunofluorescence, and mitochondrial fractionation assays. This reagent enables detailed characterization of mitochondrial protease regulation and its role in cellular energy homeostasis and disease pathology.