Anti-OATP2 (Organic Anion Transporting Polypeptide-2)

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides, oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Oatp2 (also called OATP-B1, 661 aa, 12 TM), cloned from rat brain, shares significant homology with rat oatp1 and OAT-K1 (77%) and human OATP (73%). It is highly expressed in brain, liver, and kidney but not in heart, spleen, lung, muscle, and testes. Oatp2 mediates uptake of taurocholate, estrogen conjugates, and cardiac glycosides.. Unlike other oatp's, oatp2 also transported digioxin. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 (ECL). Rat OATP2 is ~75kD in brain and liver., ELISA: 1:10,000-50,000 , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.