Anti-OATP (Organic Anion Transporting Polypeptide)

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides, oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Rat Oatp, now called oatp1, encodes a protein of 670 aa. Oatp1 was localized at the apical portions of the S3 segment of the proximal tubule, and at the apical surface of the choroid plexus of rat brain. It is also found in liver, lung, skeletal muscle, and colon. It mediates Na+-independent uptake of wide range of amphipathic, substrates including sulfobromopthalein (BSP), bile acids, estrogen conjugates, and neutral steroids, etc.. Human liver OATP (670 aa) is only 67% homologous with rat oatp1. It differs in functional properties from rat oatp1 and highly expressed in brain. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 A band of 95-110kD has been detected in brush border membrane (2)., ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml., Optimal dilutions to be determined by the researcher. Control Peptide: O3003: OATP, Human, Control Peptide. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.