Anti-NUDT8 / Nudix hydrolase 8

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. NUDT8 antibody detects Nudix hydrolase 8, a mitochondrial enzyme encoded by the NUDT8 gene on chromosome 4p14. NUDT8 belongs to the Nudix hydrolase family of pyrophosphatases, which catalyze the hydrolysis of nucleoside diphosphates and related metabolites to prevent accumulation of potentially toxic intermediates. NUDT8 is localized to the mitochondrial matrix, where it plays a role in maintaining nucleotide balance and mitochondrial integrity. It is expressed in energy-demanding tissues such as heart, skeletal muscle, and brain, reflecting its role in nucleotide metabolism and redox regulation.Structurally, NUDT8 contains the conserved Nudix (nucleoside diphosphate linked to another moiety, X) motif GX5EX7REUXEEXGU, which defines the catalytic site of the Nudix hydrolase family. The enzyme hydrolyzes substrates such as CoA diphosphate derivatives, ADP-ribose, and other nucleotide intermediates. NUDT8 belongs to the mitochondrial subfamily of Nudix enzymes, which also includes NUDT9 and NUDT15. Co-localization studies confirm its mitochondrial matrix localization, consistent with roles in energy metabolism and oxidative stress defense.Functionally, NUDT8 acts as a metabolic housekeeper by removing oxidized or excess nucleotide derivatives that can disrupt mitochondrial function. It maintains CoA and ADP-ribose homeostasis, supporting efficient energy production and mitochondrial redox balance. NUDT8 also contributes to nucleotide quality control by hydrolyzing damaged substrates generated during oxidative stress. Through this activity, it supports mitochondrial DNA replication fidelity and prevents accumulation of noncanonical nucleotides.NUDT8 expression is developmentally regulated, increasing during mitochondrial biogenesis and metabolic activation in muscle and neuronal cells. Dysregulation of NUDT8 has been linked to metabolic disorders and mitochondrial dysfunction. Reduced enzyme activity can impair oxidative phosphorylation, while overexpression enhances resilience against oxidative damage. Pathway involvement includes CoA metabolism, NAD metabolism, and nucleotide salvage pathways.The NUDT8 antibody from NSJ Bioreagents is a valuable reagent for research into mitochondrial metabolism, nucleotide regulation, and redox homeostasis.