Anti-NTN4 / Netrin 4

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. NTN4 antibody detects Netrin-4, a secreted extracellular matrix protein that guides axonal growth, angiogenesis, and tissue morphogenesis. The UniProt recommended name is Netrin-4 (NTN4). This laminin-related guidance molecule plays a dual role in promoting or inhibiting cell migration and adhesion depending on receptor context and tissue environment.Functionally, NTN4 antibody identifies a 628-amino-acid protein secreted into the extracellular space, where it binds to receptors such as UNC5B, DCC, and integrins. NTN4 regulates neuronal guidance during development by controlling axonal attraction and repulsion. Beyond the nervous system, NTN4 influences angiogenesis by modulating endothelial cell proliferation and migration, acting as both a pro- and anti-angiogenic factor in different contexts. NTN4 also contributes to basement membrane organization and vascular permeability regulation.The NTN4 gene is located on chromosome 12q22 and is expressed in brain, lung, kidney, and vascular tissues. Expression is upregulated during embryonic development, tissue regeneration, and pathological angiogenesis. NTN4 is also found in the extracellular matrix of epithelial and endothelial cells, where it interacts with laminins and collagen IV to maintain structural stability.Pathologically, altered NTN4 expression is associated with cancer, diabetic retinopathy, and neurodevelopmental disorders. Overexpression can promote tumor growth and angiogenesis, while loss of function leads to impaired vessel formation and axonal misguidance. NTN4 acts as a context-dependent regulator of cellular adhesion, guiding both neural and vascular remodeling. Research using NTN4 antibody supports studies in extracellular matrix biology, neurodevelopment, and angiogenesis.NTN4 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect secreted matrix proteins. NSJ Bioreagents provides NTN4 antibody reagents optimized for research in neural guidance, vascular development, and extracellular matrix signaling.Structurally, Netrin-4 consists of a laminin N-terminal domain (LN), three laminin-type epidermal growth factor-like repeats (LE), and a C-terminal NTR domain that mediates receptor interactions. This modular organization enables NTN4 to function as both a structural matrix component and a signaling ligand. This antibody facilitates research into NTN4's roles in neural patterning, angiogenesis, and tumor microenvironment regulation.