Anti-NSL1 / Kinetochore-associated protein NSL1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. NSL1 antibody detects Kinetochore-associated protein NSL1, a core component of the MIS12 complex required for proper chromosome segregation during mitosis. The UniProt recommended name is Kinetochore-associated protein NSL1 (NSL1). This evolutionarily conserved protein plays a crucial role in the assembly and maintenance of kinetochore structure, ensuring accurate attachment of spindle microtubules to centromeric chromatin during cell division.Functionally, NSL1 antibody identifies a 281-amino-acid nuclear and centromere-localized protein that forms part of the MIS12 complex, along with MIS12, DSN1, and PMF1. This complex acts as a central linker connecting the inner kinetochore, bound to centromeric DNA, with the outer kinetochore microtubule-binding components. NSL1 stabilizes kinetochore architecture and promotes tension-dependent microtubule attachment, which is essential for accurate chromosome alignment and segregation.The NSL1 gene is located on chromosome 1q32.3 and encodes a coiled-coil domain-containing protein that contributes to kinetochore-spindle interactions. Through its association with the MIS12 complex, NSL1 participates in recruiting key outer kinetochore proteins such as NDC80 and KNL1, forming the KMN network that mediates microtubule attachment and spindle checkpoint signaling. Disruption of NSL1 function causes chromosome mis-segregation, aneuploidy, and mitotic arrest.Beyond its structural role, NSL1 coordinates with spindle assembly checkpoint proteins to monitor proper kinetochore-microtubule tension. By serving as a molecular bridge between centromeric chromatin and spindle fibers, NSL1 ensures mitotic fidelity and genomic stability. Dysregulation or mutation of kinetochore components, including NSL1, contributes to chromosomal instability observed in cancer cells, highlighting its importance in tumor biology.NSL1 antibody is widely used in cell cycle, mitosis, and chromosome segregation research. It is suitable for immunofluorescence, immunoblotting, and immunoprecipitation to study kinetochore organization and spindle checkpoint regulation. In cancer biology, this antibody supports investigations into chromosomal instability mechanisms, mitotic defects, and checkpoint adaptation. It also aids in mapping kinetochore composition across cell cycle phases.Structurally, NSL1 contains coiled-coil motifs and conserved interaction domains that facilitate complex assembly with MIS12 and DSN1. Its function depends on dynamic phosphorylation and protein-protein interactions during metaphase and anaphase transitions. NSJ Bioreagents provides NSL1 antibody reagents validated for use in mitotic regulation, kinetochore biology, and genomic stability research.