Anti-NRCAM / Neuronal cell adhesion molecule

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. NRCAM antibody detects Neuronal cell adhesion molecule, a type I transmembrane glycoprotein belonging to the L1 family of cell adhesion molecules (CAMs). NRCAM promotes axonal guidance, synapse formation, and cell-cell communication during nervous system development. The NRCAM antibody is widely used in neuroscience, developmental biology, and neuro-oncology research to study neuronal adhesion, axonal fasciculation, and signal transduction.NRCAM is encoded by the NRCAM gene located on human chromosome 7q31.1. The protein is approximately 135 kilodaltons and comprises six immunoglobulin-like domains, five fibronectin type III repeats, a single transmembrane segment, and a short cytoplasmic tail containing ankyrin-binding motifs. These structural features allow NRCAM to interact with both extracellular ligands and intracellular cytoskeletal adaptors, linking adhesion to signal transduction and cytoskeletal organization.The NRCAM antibody detects a 135 kilodalton band by western blot and exhibits strong membrane and axonal staining in neuronal cultures and brain tissue. NRCAM mediates homophilic and heterophilic interactions with other cell adhesion molecules, including contactin and L1CAM, forming complexes that regulate neurite outgrowth, axonal pathfinding, and synapse stabilization. In developing neurons, NRCAM clusters at growth cones, coordinating adhesion with actin dynamics and microtubule extension.NRCAM also participates in signaling cascades involving the MAPK and PI3K pathways, modulating neuronal survival and differentiation. In non-neuronal tissues, NRCAM contributes to cell migration and epithelial morphogenesis, while in cancer, its overexpression promotes tumor cell adhesion and metastasis, particularly in melanoma, glioma, and prostate carcinoma.Genetic and expression studies link NRCAM variants to neurodevelopmental and psychiatric conditions, including autism spectrum disorder and schizophrenia, suggesting that altered cell adhesion signaling may underlie synaptic dysfunction. NSJ Bioreagents provides a validated NRCAM antibody optimized for its applications, enabling detailed investigation of neuronal adhesion, developmental patterning, and disease-related signaling mechanisms.