Anti-NQO-2 (DHQV, DIA6, NMOR2, QR2, Quinone Reductase 2, NADPH Quinone Acceptor Oxidoreductase 2, QR

NQO­2 (NADPH Quinone acceptor Oxidoreductase 2, also QR2, melatonin receptor 3/MT3 and NRH dehydrogenase) is a 25­26kD cytoplasmic flavoprotein member of the NAD(P)H dehydrogenase family of enzymes. It shows restricted expression, being found in retinal pigment epithelium, prostatic fibroblasts, select CNS neurons and RBCs. Like NQO­1, NQO­2 would appear to reduce catechol quinones, but without the use of NADH. In contrast to NQO­1, NQO­2 would appear to activate some quinones, generating cytotoxic products. NQO­2 also reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosome­mediated protein turnover. Finally, NQO­2 reportedly binds to melatonin, an act that may promote the subsequent binding of the androgen receptor and an inhibition of cell proliferation. Human NQO­2 is 231 amino acids (aa) in length. It contains a flavodoxin­like domain (aa4­212) and three utilized Ser phosphorylation sites. NQO­2 functions as a noncovalent homodimer. There are two potential isoform variants. One shows an alternative start site at Met117, while another shows a deletion of aa102­139. Full length human NQO­2 shares 81% aa sequence identity with mouse NQO­2, and less than 50% aa sequence identity with human NQO­1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.