Anti-NOXA2 (NCF2, p67phox, 67kD Phagocyte Oxidase, NADPH Oxidase Activator 2)

P67phox (67kD phagocyte oxidase, also NCF2 and NADPH oxidase activator 2) is a 67­70kD cytosolic member of the NCF2/NOXA1 family of proteins. It is expressed in multiple cell types, including B cells, endothelial cells, phagocytic cells such as neutrophils and macrophages and, to some degree, fibroblasts. Upon phagocytosis, phagocytes generate superoxide for the purpose of killing ingested microbes. This occurs through the formation and activation of a complex termed NADPH oxidase, which consists of membrane­associated cytochrome b558, plus Rac GTPase and three SH3 domain­containing components, p47phox and a p67phox:p40­phox dimer. Human p67phox is 526 amino acids (aa) in length. It contains two TPR repeats that participate in scaffold formation (aa11­98), an SH3 domain (aa240­299), an OPR motif that regulates PB1 interactions (aa51­429), and a C­terminal SH3 domain (aa457­516). There is one splice variant that shows a deletion of aa231­293, and multiple point mutations along the length of the molecule. Over aa355­526, human p67phox shares 76% aa identity with mouse p67phox. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.