Anti-Notch3 (Notch Homolog 3 (Drosophila), hN3, Neurogenic Locus Notch Homolog Protein 3, CADASIL, C

Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early event developmental processes. The extracellular domains of Notch receptors interact with the extracellular domains of the transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. Notch-3 has the same biochemical mechanism of signal transduction as Notch-1, where a series of cleavage events release of the Notch intracellular domain (NICD). NICD translocates to the nucleus and initiates transcription of Notch responsive genes. Thus, Notch acts as both a ligand binding receptor and a nuclear factor that regulates transcription. Human Notch-3 shows 90% aa identity to mouse Notch-3 over the entire protein. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.