Anti-Notch2 (Notch Homolog 2, Drosophila, hN2)

Anti-Notch2 (Notch Homolog 2, Drosophila, hN2)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
N5375-17.100 100 µg - -

3 - 19 business days*

901.00€
 
Human Notch-2 is a 300kD member of the notch family of transmembrane (TM) proteins. It is... more
Product information "Anti-Notch2 (Notch Homolog 2, Drosophila, hN2)"
Human Notch-2 is a 300kD member of the notch family of transmembrane (TM) proteins. It is synthesized as a 2446 amino acid (aa) type I TM glycoprotein that undergoes Golgi processing to generate a 180kD disulfide-linked extracellular domain (ECD) (likely aa25-1538) and a 110kD membrane bound truncated segment (likely aa1539-2471). Upon binding to Delta1, Jagged1, or Jagged2, the 110kD segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) (aa1697-2471), a potential nuclear transcription factor. In the nucleus, NICD regulates CSL pathways. When NICD is phosphorylated, it is deactivated. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, With an appropriate colorimetric detection system, the detection limit for rhNotch-2 ICD is ~5ng/lane under non-reducing and reducing conditions., ELISA: 0.5-1ug/ml, Detection limit for rhNotch-2 ICD is ~12ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS, and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: N5375-17

Properties

Application: ELISA, WB
Antibody Type: Monoclonal
Clone: 7H239
Conjugate: No
Host: Rat
Species reactivity: human
Immunogen: E.coli-derived recombinant human Notch-2 ICD segment (aa2063-2413), Accession # Q04721
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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