Anti-Nkx6.1 (NK homeobox factor 6.1, NKX6A)

NKX6.1 (NK homeobox factor 6.1, also NKX6A) is a 39-46kD member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a b-cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces b-cell mitosis. Human NKX6.1 is 367aa in length. It contains a repressor region composed of Pro and Ala (aa136-173), a DNA binding homeobox (aa236-295) and a Glu:Asp-rich transactivation domain (aa305-337). Over aa1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1. Applications: Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using bTC-6 mouse beta cell insulinoma cell line., Immunohistochemistry (Paraffin): 5-15ug/ml detects NKX6.1 in immersion fixed paraffin-embedded human pancreas., Immunohistochemistry (Frozen): 5-15ug/ml detects NKX6.1 in perfusion fixed frozen sections of rat pancreas., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.