Anti-Nkx 2.5 (NK-2 Family Homeodomain Transcription Factor, CSX1, Nkx2E)

NKX2-5 is a transcription factor protein which plays a crucial role in cardiac development and differentiation. It is a low molecular weight protein of 35-38kD depending on phosphorylation status. Expression is mainly restricted to the heart. But during embryogenesis NKX2-5 expression has also been detected in spleen-precursor cells. Over expression of NKX2-5 resulted in expression of neuronal markers in C2C12 murine myogenic cells. Functionally it is involved in differentiation processes in heart development and in homeostasis and survival of cardiac myocytes as well maintains the initial muscle differentiation paradigm. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.