Anti-NHE3 (Na, H Exchanger 3)

Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of Na+ and H+ ions in various tissues. The NHE catalyzes the electoneuronal transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Five isoforms (NHE3-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular. NHE3 is involved in trans-epithelial Na+ absorption. The NHE3 mRNA is found in kidney cortex, medulla, jejunum, ileum, colon and stomach (1). Applications: Suitable for use ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:100,000 Using 50-100ng control peptide/well., Western blot: 1-10ug/ml (ECL). A band of ~90kD has been detected in brush border membrane., Optimal dilutions to be determined by the researcher. Control Peptide: N2017-31: NHE3, Rat (Na, H Exchanger 3) , Storage and Stability:, May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.