Anti-NHE1 (Na, H Exchanger 1)

Na+/H+ exchangers (NHE) of mammalian cells are plasma, membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural, transport of extracellular Na+ for intracellular H+. They play a, major role in regulation of intracellular pH (pHi) in addition to, trans-cellular absorption of Na+, cell volume regulation and, possibly in cell proliferation. These primary functions of the, Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth, and hypertrophy, regression of cancer and renal intestinal, disorders. Five NHE isoforms (NHE1-5) have been cloned so, far. They are all similar in their primary structure and predicted, to have 10-12 transmembrane domains. The COOH-terminals, of NHE1, NHE2 and NHE3 are intracellular. NHE1 (rat/mouse, 820aa, human 815aa, chromosome 1 p35-36.1) is 'House Keeper' and is expressed ubiquitously in all tissues. It is implicated in pH homeostasis, volume regulation and probably cell proliferation. It is expressed on basolateral surface of several epithelia (1). Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:100,000 using 50-100ng N2015-09 Control peptide/well, Western Blot: 1:1000-1:5000 using Chemiluminescence technique. A band of 95-110kD has been detected in brush border membrane (2)., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.