Anti-NFkB, p52 (NF Kappa B, NF-kB, Nuclear Factor kappa B)

The NFkB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells. Applications: Suitable for use in Western Blot, Immunoprecipitation and Gel Shift Assay. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml. Detects NFkB p52 and its p100 precursor in HeLa nuclear extract and human Raji cell but not in mouse 3T3 cell and rat PC 12 cell lysates. , Immunoprecipitation: 4ug immunoprecipitates NF-kB p52 and its p100 precursor from 500ug HeLa nuclear extract cell lysate., Gel Shift Assay: Use 2ug per assay, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, Positive Control Included: H1838: HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.