Anti-Neuron Specific Enolase (NSE, Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Neural Enolase,

Enolase is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoenolpyruvate to phosphoenolpyruvate in the glycolytic pathway. The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma.The alpha-, or nonneuronal, enolase (ENO1) is a nearly ubiquitous form, found in almost all tissues, and its expression precedes that of the other isoforms in the early stage of embryonic development. The beta-, or muscle-specific, enolase (ENO3) is present in adult skeletal muscle, and the gamma-, or neuron-specific, enolase (ENO2) is the major form found in mature neurons and in cells of neuronal origin. Applications: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1,000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.