Anti-Neurofilament, Light Chain (NF-L)

Human neurofilament light chain (NF-L) is a 61.516 kD protein (543aa) expressed in the axons of CNS and PNS neurons, where it plays a role in maintaining the structural integrity of the axon. Mutations of the NF-L gene product are responsible for some forms of autosomal dominant Charcot-Marie-Tooth neuropathies in humans (i.e., CMT type 1F). Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. , Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues, Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells, Western Blot: 1:2000-1:5000, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch , I1906-10 IgY, Chicken (HRP) Rb x Ch , I1906-12 IgY, Chicken (FITC) Rb x Ch , I1906-14 IgY, Chicken (AP) Rb x Ch, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.