Anti-Nephronectin (NPNT, EGFL6L, POEM)

Applications:, Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200, Western Blot: 1:100-1:400, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (frozen): 1:100-1:500, Immunohistochemistry (Paraffin): 1:50-1:200, Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGHHHHHHSGSEF- LVH SCNFDHGLCG WIREKDNDLH WEPIRDPAGG QYLTVSAAKA PGGKAARLVL PLGRLMHSGD LCLSFRHKVT GLHSGTLQVF VRKHGAHGAA LWGRNGGHGW RQTQITLRGA DIKSVVFKGE KRRGHTGEIG LDDVSLKKGH CSEER, Positive Control Included: 141602A: Nephronectin, Positive Control, Suppled as a recombinant protein in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol., DAB: 10ul/well, ECL: 5ul/well, Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.