Anti-NEK9 / NIMA related kinase 9

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. NEK9 antibody detects NIMA related kinase 9, encoded by the NEK9 gene. NIMA related kinase 9 is a serine/threonine protein kinase involved in centrosome separation, spindle assembly, and mitotic progression. NEK9 antibody provides researchers with a specialized reagent for studying cell cycle regulation, mitosis, and cancer biology.NIMA related kinase 9 belongs to the NIMA related kinase family and is activated by phosphorylation at the onset of mitosis. Research using NEK9 antibody has shown that NEK9 interacts with NEK6 and NEK7, forming a kinase cascade that regulates spindle organization and microtubule stability. This cascade ensures accurate chromosome segregation and completion of mitosis, preventing genomic instability.Studies with NEK9 antibody have revealed that depletion of NEK9 disrupts centrosome separation, leading to monopolar spindle formation and mitotic arrest. These defects contribute to chromosome missegregation, aneuploidy, and cell death. The essential role of NEK9 in mitosis underscores its importance in proliferating tissues.Dysregulation of NIMA related kinase 9 has been associated with cancer, developmental disorders, and inflammatory signaling. Research using NEK9 antibody has shown that overexpression or amplification occurs in gliomas and breast cancer, promoting proliferation and survival. Mutations and loss of NEK9 function have been linked to skeletal dysplasia and microcephaly, illustrating its diverse biological significance.Beyond mitosis, research using NEK9 antibody has highlighted roles in DNA damage response and stress signaling. NEK9 phosphorylates proteins involved in checkpoint activation and stress pathways, suggesting functions beyond spindle regulation. These findings broaden its importance across cell biology.NEK9 antibody is commonly used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting quantifies kinase expression in proliferating tissues, immunohistochemistry demonstrates distribution in tumors, and immunofluorescence highlights centrosomal localization during mitosis. These applications make NEK9 antibody indispensable for cell cycle and cancer research.By providing validated NEK9 antibody reagents, NSJ Bioreagents supports studies into mitotic regulation, checkpoint signaling, and disease. Detection of NIMA related kinase 9 provides researchers with insight into how kinases regulate chromosome segregation and survival.