Anti-NEFL

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunofluorescence/ Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:200-1:400, ELISA: 1:10,000, Western Blot: 1:500-1:2000, Immunohistochemistry (Paraffin): 1:200-1:1000, Immunofluorescence (IC): 1:200-1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.