Anti-NEDD9 (Neural Precursor Cell Expressed Developmentally Down-regulated protein 9, Enhancer of Fi

NEDD9 (also HEF1 and CASL) is a 93kD (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin b1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834aa in length. It contains one SH3 domain (aa3-65), a Tyr-rich SH2-binding region (aa90-350), one Ser-rich four a-helix bundle protein interaction site (aa350-650), and an HLH motif that mediates Cas protein dimerization (aa710-760). NEDD9 usually runs at 105-115kD in SDS-PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55kD fragment. One isoform shows a deletion of aa155-188, while two others show 20-7aa substitutions for aa155-834, 1-153, respectively. Over aa153-366, human and mouse NEDD9 share 85aa identity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.