Anti-NDUFS3 / NADH dehydrogenase ubiquinone iron-sulfur protein 3

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. The NDUFS3 antibody targets NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, a core subunit of mitochondrial Complex I encoded by the NDUFS3 gene. This protein is an essential component of the oxidative phosphorylation (OXPHOS) system that catalyzes electron transfer from NADH to ubiquinone, driving ATP synthesis. NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 contributes to the assembly and stabilization of Complex I and is indispensable for mitochondrial energy metabolism. The NDUFS3 antibody enables researchers to assess mitochondrial function and respiratory chain integrity under physiological and disease conditions.Complex I, the largest enzyme complex of the electron transport chain, consists of more than 40 subunits organized into functional modules. NDUFS3 forms part of the peripheral arm that houses redox centers responsible for electron transfer. It interacts with other core subunits, including NDUFS2 and NDUFS7, during the assembly of catalytic intermediates. The NDUFS3 antibody supports detailed examination of these interactions, helping to elucidate how mitochondrial protein complexes coordinate ATP production and energy regulation.Defects in NDUFS3 are associated with mitochondrial complex I deficiency, one of the most common causes of inherited metabolic disorders. Mutations can result in severe encephalomyopathy, cardiomyopathy, or Leigh syndrome, characterized by impaired oxidative phosphorylation and reduced ATP generation. The NDUFS3 antibody is a critical tool for studying such pathologies, allowing detection of expression defects and assessing the impact of genetic variants on mitochondrial function. Immunoblotting with this antibody reveals the presence and stability of Complex I subunits in patient-derived fibroblasts and model organisms.In addition to its structural role, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 influences cellular redox balance and reactive oxygen species (ROS) generation. Dysregulation of Complex I components can contribute to oxidative stress, aging, and neurodegenerative diseases such as Parkinson's and Alzheimer's. The NDUFS3 antibody supports investigation of these mechanisms by enabling visualization of mitochondrial protein distribution and expression levels in tissues under oxidative stress or toxin exposure.The NDUFS3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It yields distinct mitochondrial staining, reflecting its subcellular localization. NSJ Bioreagents provides this antibody with high specificity and reproducibility for applications in bioenergetics, molecular biology, and pathology. By facilitating precise detection of NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, the NDUFS3 antibody supports research into mitochondrial structure, respiratory chain disorders, and mechanisms of energy homeostasis.