Anti-NDST1 / N-deacetylase/N-sulfotransferase 1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. NDST1 antibody detects N-deacetylase/N-sulfotransferase 1, an essential enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that modulates cell signaling, adhesion, and extracellular matrix organization. NDST1 catalyzes two critical reactions-N-deacetylation and N-sulfation of glucosamine residues-forming the sulfated domains that give heparan sulfate its functional specificity. The NDST1 antibody is widely used in glycobiology, developmental, and cancer research to study proteoglycan biosynthesis, cell-matrix interactions, and signaling regulation.NDST1 is encoded by the NDST1 gene located on human chromosome 5q33.1. The protein is approximately 882 amino acids long and resides in the lumen of the Golgi apparatus, where it acts early in heparan sulfate chain modification. NDST1 belongs to the heparan sulfate modification enzyme family, working in concert with other sulfotransferases and epimerases to establish specific sulfation patterns that control the binding affinity of heparan sulfate to growth factors, morphogens, and cytokines.The NDST1 antibody detects a 100 kilodalton band by western blot and demonstrates perinuclear Golgi localization under immunofluorescence microscopy. NDST1 activity is essential for embryonic development, vascular formation, and neural patterning. Through its modification of heparan sulfate, NDST1 regulates key signaling pathways including FGF, Wnt, Hedgehog, and BMP. Loss or mutation of NDST1 disrupts heparan sulfate composition, leading to developmental abnormalities and impaired growth factor signaling.In cancer, NDST1 expression is often dysregulated, resulting in altered extracellular matrix structure and aberrant cell signaling. Overexpression enhances tumor invasiveness by modifying cell-surface heparan sulfate, while reduced NDST1 activity interferes with angiogenesis. It also contributes to inflammation by modulating chemokine gradients and leukocyte migration.Because NDST1 determines the sulfation code of heparan sulfate, it serves as a critical regulator of cell communication and extracellular architecture. NSJ Bioreagents provides a validated NDST1 antibody optimized for its applications, supporting investigations into glycosaminoglycan biosynthesis, developmental regulation, and tumor microenvironment signaling.