Anti-NCLN / Nicalin

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. The NCLN antibody targets Nicalin, a membrane protein encoded by the NCLN gene that functions as a component of the Nicalin-NOMO-TMEM147 complex. This complex regulates the maturation and assembly of membrane and secretory proteins within the endoplasmic reticulum (ER). Nicalin plays a vital role in early development, cell differentiation, and signaling processes associated with protein trafficking and folding. The NCLN antibody provides researchers with a highly specific reagent to explore how Nicalin contributes to ER homeostasis and membrane protein biosynthesis.Nicalin is an integral membrane glycoprotein with several transmembrane domains that anchor it to the ER. It interacts with NOMO (Nodal modulator) and TMEM147 to form a multiprotein assembly that negatively regulates Nodal signaling, a pathway critical for embryonic patterning and left-right asymmetry. The NCLN antibody allows visualization of this protein complex and quantification of Nicalin expression in cultured cells and tissue samples, enabling insights into how ER-based regulatory systems influence developmental signaling.Beyond embryogenesis, Nicalin maintains normal ER function in adult cells by modulating protein assembly and trafficking. The NCLN antibody is particularly valuable for identifying changes in Nicalin expression during ER stress or unfolded protein response activation. These studies contribute to understanding how misfolded proteins accumulate in disease states such as neurodegeneration, metabolic disorders, and cancer. The localization of Nicalin at the ER membrane and its role in controlling protein export highlight its importance in maintaining proteostasis and cellular viability.Recent studies suggest that Nicalin may also regulate the biosynthesis of specific receptor and transporter proteins, linking it to neurotransmission and metabolic control. The NCLN antibody supports such investigations by enabling detection of Nicalin across various cell types, including neurons, epithelial cells, and hepatocytes. Its use in immunofluorescence and immunohistochemistry provides high-resolution imaging of ER organization and allows researchers to study how alterations in ER machinery affect overall cell physiology.NSJ Bioreagents provides the NCLN antibody as a validated reagent for applications including western blotting, immunofluorescence, and immunohistochemistry. It offers strong specificity for Nicalin with minimal cross-reactivity, ensuring reproducible results. By facilitating the study of this ER-resident regulatory protein, the NCLN antibody supports research into cellular protein processing, development, and signal modulation. Continued exploration using this antibody will further elucidate how Nicalin and its associated complex maintain ER balance and contribute to human health and disease.