Anti-NALP2 (NACHT-, LRR- and PYD-Containing Protein 2, Nucleotide-binding Site Protein 1, PYRIN Doma

NALP2 (NAcht Leucine-rich repeat Protein 2, also known as PAN1) is a cytosolic, 121kD member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NFkB, it suppresses TNF-alpha production in response to LPS, via procaspase-1, it promotes proIL-1-beta cleavage and release. Human NALP2 is 1062aa in length. It contains an N-terminal Pyrin domain (aa9-90), followed by a Nacht region and nine LRRs (aa467-1033). Four alternate isoforms exist. Three show deletions of aa109-132, 133-154 and 847-1062, respectively, a fourth shows a 38aa substitution for aa1-903. No close rodent counterparts have been reported. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.