Anti-NAGK / N-acetyl-D-glucosamine kinase

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. NAGK antibody detects N-acetyl-D-glucosamine kinase, encoded by the NAGK gene on chromosome 2p13.3. NAGK antibody is widely used in metabolism, glycosylation, and signaling research. NAGK is an enzyme of the hexosamine biosynthetic pathway that phosphorylates N-acetylglucosamine (GlcNAc) to GlcNAc-6-phosphate. This reaction contributes to the salvage pathway of UDP-GlcNAc biosynthesis, supporting protein glycosylation, glycosaminoglycan synthesis, and O-GlcNAc signaling.Structurally, NAGK is a ~37 kDa cytoplasmic protein belonging to the sugar kinase family. It forms homodimers and contains an ATP-binding site and catalytic residues conserved among carbohydrate kinases. NAGK is broadly expressed across tissues, reflecting its role in basic carbohydrate metabolism.Functionally, NAGK channels free GlcNAc into UDP-GlcNAc production, which is required for N- and O-linked glycosylation of proteins and for biosynthesis of glycolipids and proteoglycans. It plays a critical role in cell growth, signaling, and metabolic adaptation. Researchers use NAGK antibody to study carbohydrate metabolism, glycosylation pathways, and metabolic regulation.Clinically, altered NAGK activity is associated with metabolic diseases and cancer. Increased NAGK expression has been reported in certain tumors, where it supports growth by boosting glycosylation and nutrient sensing pathways. Because protein glycosylation is a therapeutic target, NAGK is under investigation as a biomarker and regulator of cancer metabolism. NSJ Bioreagents provides NAGK antibody for metabolism and glycosylation research.Experimentally, NAGK antibody is used in western blotting to detect the ~37 kDa enzyme, in immunohistochemistry to study tissue distribution, and in immunofluorescence to confirm cytoplasmic localization. Enzyme assays with NAGK antibody complement biochemical analyses of hexosamine pathway flux.