Anti-Myosin Light Chain 2 (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

Myosin is the major component of thick muscle filaments and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000D, and four light chains each ~16,000-21,000D. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences. Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 2.5ug/ml. Validated on a panel of 21 formalin fixed, paraffin embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with M9850-09D, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. , Western Blot: 1:500-1:2000, ELISA: 1:5000-1:20,000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.