Anti-Myogenin, clone F5D

Anti-Myogenin, clone F5D
Item number Size Datasheet Manual SDS Delivery time Quantity Price
NSJ-V3153SAF-100UG 100 µg - -

3 - 10 business days

541.00€
 
1 mg/ml in sterile PBS, BSA free, sodium azide free. Myogenin is a member of the MyoD family of... more
Product information "Anti-Myogenin, clone F5D"
1 mg/ml in sterile PBS, BSA free, sodium azide free. Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor. Protein function: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth, phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin- remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts. [The UniProt Consortium]
Keywords: Anti-MYOG, Anti-Myf-4, Anti-bHLHc3, Anti-BHLHC3, Anti-Myogenin, Anti-Myogenic factor 4, Anti-Class C basic helix-loop-helix protein 3, Myogenin Antibody
Supplier: NSJ Bioreagents
Supplier-Nr: V3153SAF-100UG

Properties

Application: ELISA, FC, IF, WB, IP, IHC
Antibody Type: Monoclonal
Clone: F5D
Conjugate: No
Host: Mouse
Reactivity: Human, Mouse, Rat, Dog, Cat, Swine
Immunogen: Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.
Format: Purified

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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