Anti-MYL6 / Myosin light chain 6

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. MYL6 antibody detects Myosin light chain 6, a regulatory component of the actin-myosin cytoskeleton that contributes to muscle contraction and cytoskeletal organization. Encoded by the MYL6 gene on chromosome 12q13.3, this protein belongs to the myosin regulatory light chain family and binds to the neck region of myosin heavy chains, stabilizing the lever arm that generates contractile force. MYL6 is expressed in both smooth and non-muscle cells, where it regulates motor activity during cell motility, cytokinesis, and tension maintenance in the actin cytoskeleton.MYL6 is one of two major isoforms produced by alternative splicing: a smooth muscle form (MYL6) and a non-muscle form (MYL6B). The smooth muscle isoform plays a key role in regulating calcium-sensitive contraction via interaction with myosin light chain kinase (MLCK) and myosin phosphatase, both of which control phosphorylation of the myosin regulatory light chain. The balance between phosphorylated and dephosphorylated MYL6 modulates smooth muscle tone and cell movement. Its structural domains include EF-hand calcium-binding motifs that enable conformational changes during contractile activation.Beyond its role in muscle contraction, MYL6 contributes to intracellular transport, cytokinetic ring formation, and endothelial barrier regulation. Alterations in myosin light chain phosphorylation have been linked to vascular diseases, asthma, and tumor metastasis. Overexpression or dysregulation of MYL6 has been observed in certain cancers and fibrotic disorders, reflecting its role in actomyosin tension and epithelial-to-mesenchymal transition (EMT). In cardiac tissue, MYL6 interacts with actin-associated proteins to influence sarcomere assembly and stress response signaling.Immunohistochemical staining using MYL6 antibody shows cytoplasmic localization in smooth muscle fibers, endothelial cells, and myofibroblasts. This antibody is widely used in research involving muscle physiology, cytoskeletal organization, and cellular contractility. MYL6 antibody from NSJ Bioreagents is suitable for studies exploring actin-myosin interactions, calcium signaling, and diseases such as hypertension, cardiomyopathy, and cancer cell invasion.