Anti-MUC5AC for IHC / Mucin 5AC, clone MSVA-109M

Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide. Mucin 5AC (MUC5AC) is a secreted gel-forming mucin predominantly expressed in gastric foveolar epithelium, where it contributes to mucosal protection, epithelial barrier integrity, and lubrication of the gastrointestinal surface. MUC5AC Antibody for IHC is specifically optimized for detection of gastric-type mucin expression in formalin-fixed, paraffin-embedded tissues, enabling high-contrast visualization of mucin-producing epithelial cells and mucin-associated tumor phenotypes in histological sections.MUC5AC antibody, also referred to as Mucin 5AC antibody or gastric mucin antibody, is widely used in immunohistochemistry as a marker of gastric differentiation and mucinous epithelial lineage. In IHC staining, MUC5AC is typically observed as strong cytoplasmic and luminal HRP-DAB brown signal within mucus-secreting epithelial cells, particularly in gastric mucosa and subsets of respiratory and gastrointestinal epithelium. Non-mucinous epithelial tissues and non-epithelial compartments such as stroma, muscle, and lymphoid tissue show minimal to absent staining, supporting its specificity for mucin-producing cell populations.Clone MSVA-109M is a recombinant mouse monoclonal antibody developed for high-affinity and reproducible detection of MUC5AC in FFPE samples. This clone produces strong, well-defined cytoplasmic staining with low non-specific background under standard antigen retrieval conditions, enabling clear identification of mucin-producing cells within complex tissue architecture. In Tissue Microarray (TMA) analysis, MUC5AC Antibody for IHC demonstrates highly consistent staining across large panels of normal and cancer tissues, allowing direct comparison of mucin expression patterns across hundreds of tissue cores within a single experimental setting.In normal tissue microarrays, MUC5AC expression is prominently detected in gastric surface epithelium and mucus-secreting glands, where intense staining highlights foveolar cells and luminal mucin. Additional expression may be observed in select respiratory epithelial cells, reflecting mucin production in airway tissues. Most other tissues, including mesenchymal and non-mucinous epithelial compartments, remain largely negative, reinforcing the lineage specificity of MUC5AC in immunohistochemistry.In cancer tissue microarrays, MUC5AC Antibody for IHC reveals strong cytoplasmic staining in a wide range of mucinous and glandular carcinomas, including gastric adenocarcinoma, colorectal adenocarcinoma, pancreatic adenocarcinoma, lung adenocarcinoma, and ovarian mucinous tumors. Tumor cells frequently exhibit increased staining intensity and expanded distribution compared to normal tissues, reflecting dysregulated mucin expression and altered differentiation states during malignant transformation. This staining pattern is particularly valuable for identifying mucinous differentiation, supporting tumor classification, and distinguishing epithelial tumor subtypes in diagnostic and research settings.The robust and reproducible staining performance of clone MSVA-109M in TMA-based immunohistochemistry supports its application in cancer research, tumor profiling, and studies of epithelial differentiation. MUC5AC Antibody for IHC enables reliable detection of mucin-producing cells in FFPE tissues and is well suited for high-throughput tissue microarray analysis, comparative pathology, and evaluation of mucin-associated tumor biology across diverse tissue types.This antibody is also part of a broader collection of IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.