Anti-MUC16 / CA125, clone CA125/8894

1 mg/ml in 1X PBS, BSA free, sodium azide free. CA125 Antibody recognizes Mucin 16 (MUC16), a high molecular weight transmembrane glycoprotein widely known as Cancer antigen 125 and Ovarian cancer antigen 125. MUC16 is a membrane-associated mucin characterized by extensive O-linked glycosylation, multiple tandem repeat domains, and a large extracellular region that can be shed into circulation. CA125 Antibody is useful for detecting MUC16 expression in epithelial and tumor tissues in research applications.MUC16 antibody, also referred to as CA125 antibody and Mucin-16 antibody in the literature, targets a mucin primarily expressed on the apical surface of epithelial cells lining the female reproductive tract, including endometrium and fallopian tube, as well as in ocular and respiratory epithelium. Under physiologic conditions, MUC16 contributes to epithelial barrier protection, lubrication, and defense against mechanical and microbial stress. Proteolytic cleavage of its extracellular domain releases circulating CA125, which forms the basis of the clinically utilized serum biomarker for ovarian carcinoma monitoring.The MUC16 gene is located on human chromosome 19p13.2 and encodes one of the largest membrane-associated proteins identified. Structurally, the protein contains numerous SEA domains, extensive tandem repeat regions, a transmembrane segment, and a short cytoplasmic tail that participates in intracellular signaling interactions. MUC16 has been shown to interact with mesothelin and other adhesion molecules, facilitating tumor cell attachment, peritoneal dissemination, and immune modulation in ovarian carcinoma.Aberrant overexpression of MUC16 is strongly associated with epithelial ovarian carcinoma and is also reported in subsets of endometrial, pancreatic, breast, and lung carcinomas. In histologic sections, MUC16 typically demonstrates apical membranous and cytoplasmic staining in positive epithelial tumor cells. A CA125 antibody is commonly applied in research settings to study epithelial differentiation, tumor origin, and mucin biology. Clone CA125/8894 is a mouse monoclonal antibody developed to target MUC16 in experimental systems.