Anti-MTMR3 / Myotubularin-related protein 3

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. MTMR3 antibody detects Myotubularin-related protein 3, a phosphatidylinositol phosphatase involved in autophagy, endosomal trafficking, and phosphoinositide metabolism. The UniProt recommended name is Myotubularin-related protein 3 (MTMR3). This enzyme belongs to the myotubularin family of dual-specificity phosphatases that dephosphorylate phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2], key regulators of endosomal dynamics and membrane trafficking.Functionally, MTMR3 antibody identifies a 911-amino-acid cytoplasmic protein containing a PH-GRAM lipid-binding domain, a catalytic phosphatase domain, and a coiled-coil region responsible for dimerization. MTMR3 acts as a negative regulator of autophagy by dephosphorylating PI3P, thereby modulating membrane dynamics at autophagosome formation sites. It also regulates endosomal sorting and vesicle trafficking between endosomes and the trans-Golgi network.The MTMR3 gene is located on chromosome 22q12.2 and is expressed in a broad range of tissues, including muscle, brain, and kidney. Its activity ensures proper membrane identity and turnover of signaling lipids within endocytic and autophagic pathways. Dysregulation of MTMR3 expression leads to aberrant phosphoinositide signaling, contributing to impaired autophagy, vesicle accumulation, and neurodegeneration.In cellular signaling, MTMR3 integrates nutrient and stress signals to balance autophagy and cell survival. It interacts with mTORC1 and ULK1 complexes, coordinating lipid metabolism with autophagic flux. Loss or inhibition of MTMR3 enhances autophagy initiation, while overexpression suppresses it. In cancer, MTMR3 acts context-dependently, functioning as either a tumor suppressor or survival factor depending on cell type and metabolic state.MTMR3 antibody is widely used in autophagy, membrane trafficking, and phosphoinositide signaling research. It is suitable for western blotting, immunofluorescence, and lipid signaling assays to detect MTMR3 expression and localization to endosomal membranes. In neurobiology, this antibody aids in studying lysosomal and endocytic dysfunction associated with neurodegenerative diseases. In cancer studies, MTMR3 detection contributes to understanding metabolic regulation and cell stress adaptation.Structurally, MTMR3 contains a catalytic Cys-X5-Arg motif typical of the protein tyrosine phosphatase superfamily, with substrate specificity directed toward phosphatidylinositol derivatives. The PH-GRAM domain mediates lipid recognition, while the coiled-coil region enables dimer formation with other myotubularin family members. NSJ Bioreagents provides MTMR3 antibody reagents validated for use in autophagy, lipid metabolism, and membrane trafficking studies.