Anti-msl-2

Make to order. Production time: 70-80 business days. Deliverables: 1. 200 µg antigen (used as positive control), 2. 1 ml pre-immune serum (used as negative control) , 3. Rabbit polyclonal antibody, antigen affinity purified. Quality Guarantee: 1. Antibody purity > 90% confirmed by SDS-PAGE, 2. Antibody titer > 1: 64,000 confirmed by ELISA, 3. WB positive with the immunogen protein (not applicable for synthetic peptide). Applications: ELISA, WB (positive for the recombinant immunogen protein/peptide). Buffer: Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Protein function: Limiting component of the male-specific lethal (MSL) histone acetyltransferase complex, a multiprotein complex essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation) (PubMed:18724933, PubMed:23084835, PubMed:23870142, PubMed:27580037, PubMed:31320325, PubMed:33208948, PubMed:7588059, PubMed:7781064, PubMed:7796814). The MSL complex specifically associates with the single X chromosome in males and mediates formation of H4K16ac, promoting a two-fold activation of X chromosome (PubMed:16543150). Msl-2 is only produced in males, constituting the limiting component of the MSL complex (PubMed:7781064). Within the MSL complex, msl-2 mediates the selective binding to the X chromosome and recruitment of the MSL complex via two different mechanisms (PubMed:18724933, PubMed:20139418, PubMed:25452275, PubMed:27580037, PubMed:31320325, PubMed:33208948, PubMed:37602401). Recognizes DNA motifs that are enriched on X chromosome, named PionX sites, which are characterized by sequence features and distinct DNA conformation (base roll) (PubMed:18724933, PubMed:25452275, PubMed:27580037, PubMed:31320325, PubMed:37602401). Specific recognition of the X chromosome is also mediated by the formation of a gel-like state: msl-2 undergoes liquid-liquid phase separation upon binding to roX1 and roX2 non-coding RNAs, leading to nucleate the MSL complex on the X chromosome (PubMed:33208948). Msl-2 is also required for translation and/or stability of msl-1 in males (PubMed:7588059, PubMed:7781064, PubMed:7796814). Also acts as an E3 ubiquitin ligase: in complex with msl-1, mediates ubiquitination of histone H2B at 'Lys-34' (H2BK34Ub) (PubMed:21726816). Also catalyzes ubiquitination of msl-1, msl-3 and mof components of the MSL complex (PubMed:23084834, PubMed:28510597). [The UniProt Consortium]