Anti-Mmp12 / Matrix metalloproteinase 12

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. MMP12 antibody detects Matrix metalloproteinase-12, also known as macrophage metalloelastase, an extracellular matrix-degrading enzyme that plays a key role in tissue remodeling, inflammation, and innate immune defense. The UniProt recommended name is Matrix metalloproteinase-12 (MMP12), and alternate names include macrophage metalloelastase, hME, macrophage elastase, and MME. MMP12 belongs to the matrix metalloproteinase (MMP) family, a group of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and regulate numerous physiological and pathological processes including wound healing, angiogenesis, and tumor progression.MMP12 is predominantly expressed in macrophages, where it contributes to tissue turnover and inflammatory regulation by cleaving elastin, fibronectin, laminin, and type IV collagen. MMP12 antibody is often used in immunohistochemistry, western blot, and zymography to assess macrophage activation and ECM degradation activity. During chronic inflammation, such as in atherosclerosis, emphysema, and rheumatoid arthritis, overproduction of MMP12 accelerates ECM breakdown and tissue destruction. Conversely, controlled MMP12 activity is necessary for effective wound repair, pathogen clearance, and extracellular remodeling in normal immune responses.Structurally, MMP12 contains the conserved catalytic zinc-binding motif (HEXXHXXGXXH) and a C-terminal hemopexin domain important for substrate specificity and interaction with tissue inhibitors of metalloproteinases (TIMPs). The MMP12 antibody is used to monitor expression and activity under various conditions where macrophage-mediated proteolysis contributes to disease pathology. The MMP12 gene is located on chromosome 11q22.2-q22.3, clustered with other MMP family members including MMP1, MMP3, and MMP13. The mature enzyme is secreted as a pro-MMP, which is activated by proteolytic cleavage or oxidative stress in inflamed tissues.Beyond matrix degradation, MMP12 regulates cytokine bioavailability and macrophage migration, influencing the balance between inflammation and resolution. Elevated MMP12 levels have been correlated with chronic obstructive pulmonary disease (COPD) severity and vascular remodeling in atheromatous plaques. Genetic knockout models in mice have shown Mmp12 deletion reduces elastin breakdown and lung injury, highlighting its central role in tissue integrity. Research using MMP12 antibody supports studies of inflammation, fibrosis, tumor invasion, and innate immunity. NSJ Bioreagents provides validated reagents that detect human, mouse, and rat MMP12 in tissues and culture systems for translational and mechanistic research.