Anti-MIF (Migration Inhibitory Factor)

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibited the random migration of macrophages. Recently, MIF was rediscovered as a pituitary hormone that acts as the counterregulatory hormone for glucocorticoid action within the immune system. MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF plays a critical role in the host control of inflammation and immunity. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells. HL­60 human acute promyelocytic leukemia cell line treated with Camphtothecin, fixed with paraformaldehyde, and permeabilized with saponin. , Western Blot: 0.5ug/ml. THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line, J774A. mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.