Anti-MICAL3 / Molecule interacting with CasL protein 3

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. MICAL3 antibody detects Microtubule-associated monooxygenase, calponin, and LIM domain-containing protein 3, an actin-modifying enzyme that regulates cytoskeletal organization and vesicle trafficking. Encoded by the MICAL3 gene on chromosome 22q11.21, this multidomain oxidoreductase functions downstream of Rab GTPases to control exocytosis and vesicle docking at the plasma membrane. MICAL3 is part of the MICAL protein family, characterized by an N-terminal flavoprotein monooxygenase domain, calponin homology domain, LIM domain, and coiled-coil regions that mediate interactions with cytoskeletal and vesicular components.MICAL3 catalyzes F-actin oxidation, leading to filament disassembly and cytoskeletal remodeling required for vesicle fusion. It interacts with Rab8A and Rab13, forming complexes that regulate polarized exocytosis and membrane trafficking in epithelial cells. MICAL3 is localized to vesicular membranes and actin-rich regions of the cytoplasm, where it coordinates actin depolymerization and vesicle release. Its enzymatic activity is dependent on flavin adenine dinucleotide (FAD) and modulates actin dynamics through redox reactions on specific methionine residues.The MICAL3 antibody is used in cell biology, neurobiology, and membrane trafficking research to study cytoskeletal regulation and exocytosis. Western blot analysis identifies a 230 kilodalton band corresponding to MICAL3, while immunofluorescence reveals punctate cytoplasmic staining in vesicle-associated regions. This antibody enables investigation of actin redox control, vesicle tethering, and neuronal growth cone dynamics.MICAL3 has been implicated in axonal transport, endocytosis, and cancer cell migration. Its deregulation may contribute to disorders involving vesicle trafficking or cytoskeletal disorganization. The MICAL3 antibody provides a validated tool for studying redox-regulated actin remodeling and membrane dynamics. NSJ Bioreagents offers this antibody optimized for western blotting and flow cytometry, ensuring high sensitivity and reproducibility in studies of cytoskeletal and vesicular processes.