Anti-MGMT, NT (MGMT, Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyl

MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Applications: Suitable for use in Western Blot, Immunofluorescence, ELISA, Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:100-500, Immunofluorescence: 1:10-50, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.