Anti-mGluR5 (Metabotropic Glutamate Receptor)

Human metabotropic glutamate receptor 5 (mGluR5, also mGluR5b) is a 150kD, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 amino acids (aa) in length and contains an extracellular domain (ECD) of 558 amino acids. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877-908 in the cytoplasmic tail. Over aa 19-509, human mGluR5 is 98% aa identical to mouse, rat and canine mGluR5. Applications: Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhmGluR5 is ~ 0.2ng/well. , Western Blot: 0.1-0.2ug/ml detection limit for rhmGluR5 is ~ 10ng/lane and 1ng/lane under non-reducing and reducing conditions, respectively. , Immunohistochemistry: 2-5ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.