Anti-mGluR5 (Metabotropic Glutamate Receptor)

Metabotropic glutamate receptors have been classified into three groups. In general, class I receptors (mGluR1 and mGluR5) mediate the excitation of neurons, whereas activation of class II (mGluR2 and mGluR3) and class III (mGluR4a, mGluR7a, mGluR7b and mGluR8) results in a depression of synaptic transmission. Class I mGluRs are localized in the postsynaptic membrane and are positively coupled to phospholipase C (PLC). Class II and class III mGluRs localize to the presynaptic membrane and are negatively coupled to adenylate cyclase. It has been speculated that mGluR2 and/or mGluR3 may play a role in the modulation of the mossy terminal function. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immuncytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml (ECL) on rat brain microsomal preparation. Detects a band of ~132kD., Immunohistochemistry: 0.1-0.2ug/ml. Rat and mouse brain sections fixed with 0.1M PBS, pH 7.4, 4% paraformaldehyde, 15% saturated picric acid. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. M3884-79D was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed. Slides were incubated overnight at 4ºC with diluted M3884-79D. Slides were rinsed 3 times. Slides were incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy., Immunocytochemistry: 0.5ug/ml, Immunofluorescence: Indirect, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.