Anti-Mesothelin / MSLN, clone MSVA-235M

Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide. Mesothelin (MSLN) is a glycosylphosphatidylinositol-anchored cell surface protein selectively expressed on mesothelial cells and overexpressed in several epithelial malignancies. It is widely used as a marker of mesothelial differentiation and tumor-associated antigen in diagnostic immunohistochemistry. Mesothelin Antibody for IHC enables sensitive and specific detection of MSLN expression in formalin-fixed, paraffin-embedded (FFPE) tissues, supporting evaluation of mesothelial lineage and identification of mesothelin-positive tumors.In immunohistochemistry, mesothelin antibody, also known as MSLN antibody or mesothelioma marker antibody, produces distinct membranous and cytoplasmic HRP-DAB brown staining corresponding to cell surface localization and protein processing. In FFPE human tissue microarrays (TMAs), strong and reproducible staining is consistently observed in mesothelial linings and mesothelin-expressing epithelial tissues, while the majority of non-mesothelial tissues demonstrate minimal to absent staining. The use of large-scale TMA panels highlights high specificity, low background, and consistent staining performance across diverse tissue types.Mesothelin Antibody for IHC is particularly valuable in cancer tissue microarray analysis, where it enables robust identification of mesothelin-expressing malignancies. In TMA panels containing a broad range of tumors, strong membranous staining is observed in mesothelioma as well as ovarian serous carcinoma and pancreatic adenocarcinoma, reflecting known patterns of mesothelin overexpression. In contrast, most non-expressing tumor types show little to no staining, providing clear contrast that supports tumor classification and aids in distinguishing mesothelioma from other malignancies with overlapping morphology.The reproducible staining patterns observed across FFPE TMAs support reliable comparison of expression across normal and malignant tissues. The combined membranous and cytoplasmic staining pattern enhances visualization of tumor cell populations and mesothelial structures, while minimal non-specific staining improves interpretability in complex tissue environments. These characteristics are particularly important for high-throughput immunohistochemistry studies and comparative biomarker analysis.Functionally, mesothelin contributes to cell adhesion and tumor progression, including interactions with MUC16 (CA125) that promote tumor cell dissemination. Its restricted expression in normal tissues and elevated expression in multiple cancers underpin its diagnostic and therapeutic relevance. Overall, Mesothelin Antibody for IHC provides robust, high-contrast staining in FFPE tissue microarrays, supporting accurate identification of mesothelial differentiation and reliable detection of mesothelin-positive tumors.This antibody is also part of a broader collection of IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.This Mesothelin antibody is also part of a broader Mesothelin antibody panel offered by NSJ Bioreagents.