Anti-MCM7 / Minichromosome maintenance complex component 7, clone MSVA-507R

Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide. Minichromosome maintenance protein 7 (MCM7) is a nuclear DNA replication factor encoded by the MCM7 gene and functions as a core component of the minichromosome maintenance helicase complex responsible for eukaryotic DNA replication. The MCM7 Antibody for IHC (clone MSVA-507R) recognizes this replication licensing protein and is designed for immunohistochemistry analysis of proliferating cells in normal and neoplastic tissues. Because MCM7 expression is closely linked to DNA synthesis and cell cycle progression, immunohistochemistry staining patterns provide valuable insight into proliferative activity within tissue sections. This antibody is part of a broader collection of IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.MCM7 belongs to the conserved MCM protein family that forms the heterohexameric MCM2-7 helicase complex. This complex assembles at replication origins during the G1 phase of the cell cycle as part of the pre-replication complex that licenses chromatin for DNA synthesis. Activation of the helicase during S phase allows separation of double-stranded DNA at replication forks, enabling DNA polymerases and associated replication machinery to duplicate the genome. Because of this role in DNA replication licensing, nuclear expression of MCM7 is typically observed in actively cycling cells.MCM7 antibody, also referred to as CDC47 antibody and P1-MCM3 antibody in the literature, detects a nuclear protein that is widely used as an immunohistochemical marker of cellular proliferation. In immunohistochemistry studies, MCM7 staining typically appears as nuclear HRP-DAB brown signal in proliferating cell populations. In normal tissues, staining is often restricted to proliferative compartments such as germinal center lymphocytes, basal epithelial layers, intestinal crypt cells, and other regions containing actively dividing cells, while differentiated cells generally show reduced or absent signal.Immunohistochemistry studies have also shown elevated MCM7 expression in a broad range of cancers including colorectal carcinoma, breast carcinoma, lung carcinoma, and other rapidly proliferating malignancies. In tumor samples, strong nuclear staining frequently highlights proliferating tumor cell populations and can be observed in both glandular and solid tumor architectures. These patterns reflect the increased DNA replication activity that characterizes malignant cell growth.Testing of MCM7 Antibody for IHC (clone MSVA-507R), a recombinant rabbit monoclonal antibody, across a wide panel of normal and cancer tissue microarrays demonstrates staining patterns consistent with published expression data, including datasets such as the Human Protein Atlas. The antibody shows nuclear staining in proliferating cell populations across multiple tissue types and tumor samples, supporting its use in immunohistochemistry-based studies examining cell proliferation, tumor biology, and DNA replication licensing mechanisms.This antibody is part of a broader panel of MCM7 antibodies designed to support detection of proliferation-associated proteins across multiple research applications.