Anti-MCAM (Cell Surface Glycoprotein MUC18, Melanoma-associated Antigen MUC18, Melanoma Cell Adhesio

MCAM (Melanoma cell adhesion molecule, also CD146 and MUC18) is a 110-120kD member of a small group of Ig-superfamily molecules that includes CD239 and CD166. MCAM has also been reported at a molecular weight of approximately 150kD. In rodent, MCAM is reportedly expressed on neurons, endothelial cells, NK cells, neutrophils, mesenchymal stem cells and melanoma cells. MCAM appears to contribute to intercellular endothelial cell junctions, and possibly contributes to the migration of select cell types. Mature mouse MCAM is a 625aa type I transmembrane glycoprotein. Its extracellular region is 540aa in length (aa24-563). It contains two V-type Ig-like domains (aa24-244) followed by three C2-type Ig-like domains (aa246-512). One cytoplasmic region splice form shows a sevenaa substitution for aa600-648. Unlike human, rodent MCAM does not undergo a splicing event that will generate a soluble isoform. Applications: Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using bowes human melanoma cell line and mouse embryo tissue, Immunocytochemistry: 5-15ug/ml using immersion fixed B16-F1 mouse melanoma cell line, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.