Anti-MARCO (SCARA2, Macrophage Receptor with Collagenous Structure, Scavenger Receptor Class A Membe

MARCO (Macrophage receptor with collagenous structure, also scavenger receptor class A member 2/SR­AII) is a 65­70kD pattern recognition receptor/PRR that belongs to class A of the SR family, SRCR superfamily of molecules. It is expressed by macrophages, monocytes and Kupffer cells, and apparently binds to a number of microbes, including Gram+ bacteria (Staph and Strep) and Gram­ bacteria (Neisseria and coliforms), plus crystalline silica. Human MARCO is a 520 amino acid (aa) type II tranamembrane glycoprotein. It contains a 43aa cytoplasmic region and a 456aa extracellular domain/ECD (aa65­520). The ECD possesses one collagen­like region (aa147­419) and an SRCR domain (aa424­519). Human MARCO is believed to form trimers and/or multimers, and these are likely noncovalent associations. Over aa79­520, human and mouse MARCO share 70% aa sequence identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.