Anti-MAP1LC3B/LC3B (N-Term) Rabbit Monoclonal Antibody (Clone RM293)

Microtubule-associated protein 1 light chain beta (MAP1LC3B), also known as LC3B, is an ortholog of yeast Atg8 that is essential to autophagosome biogenesis. LC3B is irreversibly cleaved by the cysteine protease ATG4B (Cay-25380) to its active form LC3B-I, which contains an exposed C-terminal glycine residue needed for autophagosome formation. LC3B-I is then conjugated to phosphatidylethanolamine in canonical autophagy by the ATG12-ATG5-ATG16 complex to form LC3B-II, which localizes to the autophagosomal membrane. Knockdown of LC3B increases the number of ATG5- and ATG16-positive puncta in HeLa cells, indicating a defect in the maturation of autophagosomes. LC3B expression is upregulated in various cancers and has disease-specific implications on progression and prognosis. In metastatic and castration-resistant human prostate cancer tumor samples, LC3B expression is upregulated and is positively correlated with reduced disease-specific mortality. Expression of LCB3 is also increased in oral squamous cell carcinomas and is associated with aggressive pathological features and poor prognosis. Cayman's MAP1LC3B/LC3B (N-Term) Rabbit Monoclonal Antibody (Clone RM293) can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications.Synonyms: Autophagy-related Protein LC3B, Autophagy-related Ubiquitin-like Modifier LC3B, MAP1 Light Chain 3-like Protein 2, Microtubule-associated Protein 1 Light Chain 3beta. Immunogen: A peptide from the N-terminal region of human LC3B. Clone Designation: RM293. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody.. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ICC, IHC, WB. Cross Reactivity: (+) LCB3.