Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) (ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1,

In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome. UniProt Number: Human(P28482), Mouse(P63085), Rat(P63086), Human(P27361), Mouse(Q63844), Rat(P21708), Gene ID: MAPK1, MAPK3 , Applications: Suitable for use in Western Blot, Immunohistochemistry (Paraffin), and Immunocytochemistry. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Handling: Store at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term., Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.