Anti-MAML3 / Mastermind-like protein 3

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. The MAML3 antibody targets Mastermind-like protein 3, a transcriptional coactivator encoded by the MAML3 gene. This protein is a member of the Mastermind-like family, which serves as a key nuclear coactivator in the Notch signaling pathway. Mastermind-like protein 3 interacts with the intracellular domain of Notch receptors and the transcription factor CSL (CBF1/RBPJ) to assemble transcriptional activation complexes that regulate cell fate decisions, proliferation, and differentiation. The MAML3 antibody provides researchers with a powerful tool for studying Notch-dependent transcriptional control and its roles in development and oncogenesis.Mastermind-like protein 3 acts as a scaffold that stabilizes the Notch-CSL complex on target promoters, recruiting additional coactivators such as p300/CBP to initiate transcription. Its activation leads to expression of Notch target genes, including those involved in stem cell maintenance, neural development, and tumorigenesis. The MAML3 antibody supports studies aimed at elucidating these transcriptional networks by allowing detection and localization of MAML3 in nuclear extracts and tissue sections.Chromosomal rearrangements involving MAML3 have been implicated in human cancers, notably biphenotypic sinonasal sarcoma and certain neuroendocrine tumors. The resulting fusion proteins, such as PAX3-MAML3, act as potent transcriptional activators driving oncogenic programs. The MAML3 antibody enables researchers to analyze wild-type and fusion variants, supporting investigations into the molecular mechanisms by which Mastermind-like protein 3 contributes to tumor development and aberrant Notch signaling.Beyond its canonical Notch-related functions, Mastermind-like protein 3 participates in other signaling pathways, including Wnt and Hedgehog, where it modulates transcriptional responses through interactions with diverse transcription factors. The MAML3 antibody aids in identifying these alternative regulatory roles, helping to map MAML3-containing transcriptional complexes in various tissues and developmental stages. Expression analysis indicates enrichment of MAML3 in neural and mesenchymal lineages, consistent with its role in differentiation control.NSJ Bioreagents provides the MAML3 antibody as a high-specificity reagent for western blotting, immunohistochemistry, and immunofluorescence. It consistently detects Mastermind-like protein 3 with strong nuclear localization. By enabling exploration of transcriptional coactivation mechanisms and Notch signaling regulation, the MAML3 antibody supports fundamental and translational research into cell fate determination, cancer, and developmental biology.